Device
Lysis

Part:BBa_K836009

Designed by: Ricardo Alvarado   Group: iGEM12_Costa_Rica-TEC-UNA   (2012-09-20)


Lysis device for R. opacus

Lysis construct based on the one presented by Berkeley's 2008 team BBa_K112022 (https://parts.igem.org/wiki/index.php?title=Part:BBa_K112022. It is based on the inducible expression of a lysozyme and lambda phage's holin in order to liberate internal products of the bacterial cell in an efficient way. The induction is achieved by the addition of nitrile or a similar agent since both genes are regulated by nitA promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2212
    Illegal BamHI site found at 994
    Illegal XhoI site found at 3156
    Illegal XhoI site found at 3312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2790
    Illegal NgoMIV site found at 3048
    Illegal NgoMIV site found at 3349
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1753
    Illegal BsaI site found at 2561
    Illegal BsaI site found at 3008
    Illegal BsaI site found at 3485
    Illegal BsaI.rc site found at 410
    Illegal BsaI.rc site found at 710
    Illegal BsaI.rc site found at 983
    Illegal BsaI.rc site found at 1150
    Illegal BsaI.rc site found at 1666
    Illegal SapI site found at 1221


[edit]
Categories
//cds/lysis
Parameters
None